I comply with acquire foreseeable future electronic communications from STEMCELL. I admit that I can unsubscribe any time.
As soon as the operate is complete, retrieve the Shipping Cartridge within the instrument. The cell sample ought to be at the bottom or aspect of the collection tube. Note: It is suggested to spin down the Shipping Cartridge in the mini-centrifuge for any few seconds for whole quantity Restoration.
Circulation cytometry can be used to evaluate the performance of differentiation protocols With all the STEMdiff™ system, by thinking about unique marker expression at Every single phase in the differentiation process. It can be useful for other programs, which include cell sorting, immunophenotyping, and purity assessment.
It is necessary to quench the dissociation reagent working with FACS buffer or stop medium, by incorporating a minimum of precisely the same or double the amount with the dissociation reagent.
Cross-area histology accompanied by hematoxylin and eosin (H&E) staining to evaluate the thickness with the small or significant airway epithelium
Make additional informed paying for selections with our new item availability and shipping estimate characteristic, now out there on all solution web pages, in the cart, And through checkout.
There are several commercially accessible resources for Major airway epithelial cells. Now we have examined HAECs from two sellers, Lonza and Epithelix — both of those have resulted in profitable ALI cultures.
Be aware: Commence to antibody staining if undertaking Are living cell analysis following this stage. Alternatively, move forward to fixable dye staining and PFA fixation.
In case cell clumping is observed right after thawing cryopreserved cells, it is usually recommended to filter aggregated suspensions through a 37 µm cell strainer (e.g. Catalog #27250) for ideal benefits. In such cases, the run time 지방흡입 over the CellPore™ Transfection System may well need to be improved to ten seconds.
To evaluate the regional specificity from the modest vs huge airway, you'll be able to carry out the subsequent 지방흡입 assays:
I conform to acquire upcoming Digital communications from STEMCELL. I admit which i can unsubscribe any time.
Make a lot more knowledgeable paying for decisions with our new solution availability and shipping estimate aspect, now available on all solution web pages, as part of your cart, And through checkout.
Be 줄기세포 지방이식 aware: PFA fixation might be regarded for evaluating intracellular markers or doing Examination at a later time.
Quickly thaw cells in a very Stem cell 37°C water bathtub by gently shaking the cryovial. Take 지방이식 out the vial when a small frozen cell pellet continues to be. Be aware: It is important to work quickly in the next steps to be sure higher cell viability and Restoration.